Recycling of 5′-nucleotidase in a rat hepatoma cell line.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Pyridoxine resistance in a rat hepatoma cell line.

The natural vitamin, pyridoxine, in the millimolar range is toxic to cultured rat hepatoma cells. A pyridoxine-resistant Fu5-5 rat hepatoma cell line was established by a stepwise increase in the concentration of pyridoxine in the medium. The newly established cell line, referred to as clone 10 (Cl.10), is resistant to killing by pyridoxine in concentrations up to 5 mM. Saturation kinetics for ...

متن کامل

5'-Nucleotidase of rat liver microsomes.

Recent observations from this laboratory have revealed that there is an alteration in the kinetic properties of microsomal glucose 6-phosphatase in the livers of diabetic rats (1, Z), in addition to the’well established increase in total activity (3, 4). Since the kinetic differences between the normal and the diabetic enzyme disappeared upon disruption of the microsomes (Z), it seemed possible...

متن کامل

Cytotoxicity of Subtoxic AgNP in Human Hepatoma Cell Line (HepG2) after Long-Term Exposure

Background: We aimed at evaluating the toxicity effects of low (subtoxic) concentrations of silver nanoparticles (AgNP, 5-10 nm) in human hepatoblastoma (HepG2) cell line after and during a period of about one month. Methods: XTT and MTT assays were used to draw a dose-response curve IC50 (half maximal inhibitory concentration) value of the AgNP on HepG2 cells was calculated to be 2.75-3.0 mg/l...

متن کامل

Antioxidant Effects of Pinus Eldarica Extracts Against Cisplatin-Induced Cytotoxicity in Human Hepatoma (Hepg2) Cell Line

Background:  Cisplatin has cytotoxic effects through free radical generation. Reports indicate that various members of Pinaceae family may have antioxidant properties. In this study we investigated the cytoprotective effects of needle volatile oil and bark extract of Pinus eldarica in hepatoma G2 (HepG2) cell line.  Methods: Using the maceration technique, we obtained the ethanolic extract of ...

متن کامل

A soluble 5'-nucleotidase in rat kidney. Stimulation by decavanadate.

A soluble 5'-nucleotidase was identified in rat kidney and partially purified. Compared with 5'-IMP, 5'-AMP was a poor substrate. The affinity for 5'-IMP was very low (S0.5 greater than 1 mM) in the absence of an activator, and it was much increased (S0.5 = 0.1 mM) by 2,3-bisphosphoglycerate (2,3-DPG). ATP and bisadenosyl tetraphosphate were further activators. The pH optimum was 6.3. Those pro...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: The EMBO Journal

سال: 1988

ISSN: 0261-4189

DOI: 10.1002/j.1460-2075.1988.tb03206.x